Analysis of histone post translational modifications in primary monocyte derived macrophages using reverse phase×reverse phase chromatography in conjunction with porous graphitic carbon stationary phase
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چکیده
منابع مشابه
Analysis of histone post translational modifications in primary monocyte derived macrophages using reverse phase × reverse phase chromatography in conjunction with porous graphitic carbon stationary phase
A two dimensional-liquid chromatography (2D-LC) based approach was developed for the identification and quantification of histone post translational modifications in conjunction with mass spectrometry analysis. Using a bottom-up strategy, offline 2D-LC was developed using reverse phase chromatography. A porous graphitic carbon stationary phase in the first dimension and a C18 stationary phase i...
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istry 204: 215}219. Chaplin MF and Kennedy JF (eds) (1994) Carbohydrate Analysis:A Practical Approach, 2nd edn, pp. 15, 41, 66, 152, 209. Oxford: IRL Press. Charlwood J, Birrell H, Tolson D and Camilleri P (1998) Two-dimensional chromatography in the analysis of complex glycans from transferrin. Analytical Chemistry 70: 2530}2535. Guille GR, Rudd PM, Wing DR, Prime SB and Dwek RA (1996) A rapid...
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Understanding the Complexity of Porous Graphitic Carbon (PGC) Chromatography: Modulation of Mobile-Stationary Phase Interactions Overcomes Loss of Retention and Reduces Variability
Porous graphitic carbon (PGC) is an important tool in a chromatographer's armory that retains polar compounds with mass spectrometry (MS)-compatible solvents. However, its applicability is severely limited by an unpredictable loss of retention, which can be attributed to contamination. The solutions offered fail to restore the original retention and our observations of retention time shifts of ...
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Nucleosomes are the smallest structural unit of chromatin, composed of 147 base pairs of DNA wrapped around an octamer of histone proteins. Histone function is mediated by extensive post-translational modification by a myriad of nuclear proteins. These modifications are critical for nuclear integrity as they regulate chromatin structure and recruit enzymes involved in gene regulation, DNA repai...
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ژورنال
عنوان ژورنال: Journal of Chromatography A
سال: 2016
ISSN: 0021-9673
DOI: 10.1016/j.chroma.2016.05.025